SEED SPECIFIC PROMOTER ISOLATION

Omega-9 Desaturase Gene Expression in Oil seeds

The omega-9 desaturase enzyme(stearoyl ACP desaturase) is involved in the oil synthesis pathway in oil seeds.

In peanut and sunflower seeds, the omega - 9 desaturase transcript accumulate highly in the early stages of seed development. (Ref: 9, 10, 11)

 

Peanut(1.545kb)					A	B	Ratio
				Stage 1			2.8
				Stage 2			2.3
				Stage 3			1.3
1	2	3	4	Leaf			1.5

 

	Sun Flower
	N	N	HO	N	HO	N
10 DAP							1.7 kb
16 DAP							1.7 kb
20 DAP

 

The omega- 9 desaturase promoter is putative seed specific promotor.

We have isolated a peanut omega - 9 desaturase promotor that we are currently characterizing.

This promotor will be fused to the fiber synthetic genes for large scale production of fiber proteins in seed storage protein producing plants.