HETEROPOLYMERS

Research Strategy

Results

We have engineered collagen-spidroin 2 heteropolymeric clones of different sizes using a PCR based method. A synthetic heteropolymeric gene of 587 bp was made and introduced into yeast. Western blot analysis (Figure 2) shows the presence of the copolymer protein into the culture media.

This synthetic heteropolymeric gene has been moved into a plant expression vector for transgenic expression in tobacco protoplasts.

The recombinant proteins were produced in recombinant yeasts and detected at different time points after induction of protein synthesis.

Figure 2: Western-blot showing the synthetic collagen-spidroin 2protein*